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iPSC Culture Protocol Videos
Watch our iPSC culture protocol videos听to learn how to culture iPSCs and iPSC-derived neural cells.
English, French, Portuguese, and Spanish videos are available now, with more languages coming soon!
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At the EDDU, we developed a method with microfabricated disk (EB disk-948 from eNUVIO) to scale up the generation of hMOs (human midbrain organoids). This opens up the possibility to generate larger numbers of hMOs, in a manner that minimizes the amount of labor required, while decreasing variability and maintaining the viability of these hMOs over time.
This video will describe the methodology to generate hMOs from a single EB-Disk containing 948 microwells.
The Neuro鈥檚 EDDU is also pleased to share our updated 鈥痯rotocol for generating dopaminergic neurons from鈥� iPSCs through the use of听a鈥� Microfabricated EB Disk.听You can access the protocol here:听
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Latest Publications
To access all of the EDDU's publications, please visit our听.听
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CelltypeR: A flow cytometry pipeline to characterize single cells from brain organoids
July 2024
Rhalena A. Thomas et al.听
An Inducible Luminescent System to Explore Parkinson鈥檚 Disease-Associated Genes
August 2024
Anelya Gandy et al.
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Preprints
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Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures
December 2024
Gabriela J听Blaszczyk et al.
Pro-Inflammatory Molecules Implicated in Multiple Sclerosis Divert the Development of Human Oligodendrocyte Lineage Cells
December 2024
Gabriela J Blaszczyk et al.
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How to Access iPSCs
At the EDDU we have a catalogue of iPSC lines from healthy individuals and from patients with neurological disorders that can be freely accessed for a small fee. Our cells are for experimental use only.
How to access the iPSC catalogue:
- Click 鈥淥pen data access鈥� as login
- Select at the top-left 鈥淥pen data鈥� tab
- Click 鈥渋PSC catalogue鈥�
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To access the cells, please cbig.mni [at] mcgill.ca (C-BIG). Click for further instructions on accessing cells.
To learn more about how the cells are made or for general inquiries, please email xiuqing.chen [at] mcgill.ca (Carol Chen) and taylor.goldsmith [at] mcgill.ca ( Taylor Goldsmith).
Visit our Community and Resource Hub
To access the EDDU鈥檚 Open Science resources including our听publications, presentations, protocols, and software听please visit our Community and Resource Hub.
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